Staurosporine

Staurosporine is a natural product originally isolated in 1977 from the bacterium Streptomyces staurosporeus by Omura et al . It was the first of over 50 alkaloids to be isolated with this type of chemical structure. The chemical structure of Staurosporine was elucidated by X-ray analysis of a single crystal and the absolute stereochemical configuration by the same method in 1994 .

Staurosporine was discovered to have biological activities ranging from anti-fungal to anti-hypertensive. The interest in these activities resulted in a large investigative effort in chemistry and biology and the discovery of the potential for anti-cancer treatment. This led to the chemical synthesis of Staurosporine itself followed by the synthesis of compounds that had increasingly diverse chemical structure and their biological assessment.

Staurosporine is a potent inhibitor of many, many kinases including protein kinase C (IC50 = 0.7 nM), protein kinase A (IC50 = 7 nM), and protein kinase G (IC50 = 8.5 nM). Matsumoto, H. and Sasaki, Y. "Staurosporine, a protein kinase C inhibitor, interferes with proliferation of arterial smooth muscle cells." Biochem. Biophys. Res. Commun. 158: 105-109 (1989). Tamaoki, T., et al. "Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase." Biochem. Biophys. Res. Commun. 135: 397-402 (1986).

Staurosporine induces apoptosis in human neuroblastoma cell lines and chick embryonic neurons. Boix, J., et al. "Characterization of the cell death process induced by staurosporine in human neuroblastoma cell lines." Neuropharmacology 36: 811-821 (1997). Wiesner, D.A. and Dawson, G., "Staurosporine induces programmed cell death in embryonic neurons and activation of the ceramide pathway." J. Neurochem. 66: 1418-1425 (1996).
------------------

Induction of Apoptosis by Treatment with Staurosporine

Staurosporine (Streptomyces staurospores) is a relatively non-selective protein kinase inhibitor, which blocks many kinases to different degrees. Staurosporine is often used as a general method for inducing apoptosis.

Protocol for Staurosporine-induced Apoptosis:

Add 1 mM (final concentration) staurosporine (eg, Sigma S6942) to cell suspension (eg, 5 x 10⁵ cells/ml in tissue culture medium).
Perform a time course to obtain optimum results; a 1-6 hr incubation at 37°C is suggested.
Proceed with assays designed to evaluate induction of apoptosis.

-------------

source: Kabir J, Lobo M, Zachary I. 2002 Staurosporine induces endothelial cell apoptosis via focal adhesion kinase dephosphorylation and focal adhesion disassembly independent of focal adhesion kinase proteolysis. J. Biochem. 367: 145-155.

Disclaimer:

This page was made by the students of bioinformatics, the OpenUniversity, BirZeit P.A., as a homework in the Survival in the iJungle. It has no intelectual value whatsoever.

If you think your copyright has been violated, we shall remove the offending content at once. Please eMail to: Abuse17@biochem-openu.cjb.net and start your SUBJECT field with the word Abuse17 (Case sensitive) that it might penetrate our spam protection.

HABASAR

Links of the YOLL itself
We must have at least 100 pages here.

Here come some links to top-rated hrefs, such as Microsoft, wikipedia, Google and Yahoo :google, microsoft, yahoo, wikipedia

Now come links to these of fermentek product pages, that are needy : .the homepage of fermentek, FK506 or tacrolimus, products of Fermentek: staurosporine, K252A, aflatoxin, Sirolimus (Rapamycin)

And other link beggars:

Tinnitus. about some guys that had tinnitus

Nonactin at noneto. Some facts about nonactin. Why nobody needs it. Who is prof Nigel mister nonactin, and what is his role in promiliad